荧光染料Dye Reagents BODIPY-Cholesterol; 纯度: ge;98.0%
BODIPY-cholesterol 是一种带有荧光 BODIPY 基团的亲脂性和细胞通透性胆固醇类似物。BODIPY-cholesterol 可用于监测细胞内固醇摄取和细胞器间固醇通量。(激发/发射波长: 480/508 nm)。
BODIPY-Cholesterol Chemical Structure
CAS No. : 878557-19-8
规格 | 价格 | 是否有货 | 数量 |
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1 mg | ¥4000 | In-stock | |
5 mg | ¥9500 | In-stock | |
10 mg | ; | 询价 | ; |
50 mg | ; | 询价 | ; |
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BODIPY-Cholesterol 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
生物活性 |
BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm)[1]. |
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体外研究 (In Vitro) |
BODIPY-cholesterol assayTo prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM[1].Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration[1].Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 µM), mixed thoroughly and incubated for 10 -20 mins at 37 °C[1].The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
576.61 |
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Formula |
C36H51BF2N2O |
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CAS 号 |
878557-19-8 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
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溶解性数据 |
In Vitro:;
DMSO : 1 mg/mL (1.73 mM; Need ultrasonic and warming) H2O : < 0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble) 配制储备液
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
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参考文献 |
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