Nile Red(Synonyms: 尼罗红 Nile Blue A oxazone Phenoxazone 9)

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Nile Red;(Synonyms: 尼罗红; Nile Blue A oxazone; Phenoxazone 9) 纯度: 98.02%

Nile Red (Nile Blue A oxazone) 是一种针对细胞内脂质小滴和中性脂质的选择性疏水性荧光染料。Nile Red 在所有有机溶剂中均具有强烈的荧光性，其荧光颜色范围从金黄色到深红色。

Nile Redamp;;(Synonyms: 尼罗红; Nile Blue A oxazone; Phenoxazone 9)

Nile Red Chemical Structure

CAS No. : 7385-67-3

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥440 In-stock
10 mg ￥400 In-stock
50 mg ￥650 In-stock
100 mg ￥800 In-stock
500 mg ￥1400 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

Nile Red 相关产品

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  • Bioactive Compound Library Plus

生物活性

Nile Red (Nile Blue A oxazone) is a selective and hydrophobic fluorescent stain for intracellular lipid droplets and neutral lipids. Nile Red is intensely fluorescent in all organic solvents and the fluorescence colors range from golden yellow to deep red[1][2].

体外研究
(In Vitro)

Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm)[1].
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution[1].
Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal’s genotype or experimental treatment[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

318.37

Formula

C20H18N2O2
CAS 号

7385-67-3
中文名称

尼罗红
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : ≥ 50 mg/mL (157.05 mM)

H2O : 0.1 mg/mL (0.31 mM; Need ultrasonic)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.1410 mL 15.7050 mL 31.4100 mL
5 mM 0.6282 mL 3.1410 mL 6.2820 mL
10 mM 0.3141 mL 1.5705 mL 3.1410 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Greenspan P, et al. Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol. 1985 Mar;100(3):965-73.

    [2]. Gibrán S Alemán-Nava, et al. How to use Nile Red, a selective fluorescent stain for microalgal neutral lipids. J Microbiol Methods. 2016 Sep;128:74-79.

    [3]. Wilber Escorcia, et al. Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining. J Vis Exp. 2018 Mar 5;(133):57352.

    [4]. Elizabeth C Pino, et al. Biochemical and high throughput microscopic assessment of fat mass in Caenorhabditis elegans. J Vis Exp. 2013 Mar 30;(73):50180.