Thioflavin T;(Synonyms: 硫黄素T; Basic Yellow 1) 纯度: 98.72%
Thioflavin T 是一种阳离子 Benzothiazole 染料,在组织切片中与淀粉样蛋白结合后荧光强度增强。
Thioflavin T Chemical Structure
CAS No. : 2390-54-7
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10;mM;*;1 mL in DMSO | ¥550 | In-stock | |
500 mg | ¥500 | In-stock | |
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Thioflavin T 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
生物活性 |
Thioflavin T is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections. |
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体外研究 (In Vitro) |
Thioflavin T (ThT) is a benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils and is commonly used to diagnose amyloid fibrils, both ex vivo and in vitro. In aqueous solutions, Thioflavin T is found to exist as micelles at concentrations commonly used to monitor fibrils by fluorescence assay (~10-20 μM). Specific conductivity changes are measured at varying concentration of Thioflavin T and the critical micellar concentration is calculated to be 4.0±0.5 μM. Changes in the fluorescence excitation and emission of Thioflavin T are also dependent on the micelle formation. The Thioflavin T micelles of 3 nm diameter are directly visualized using atomic force microscopy, and bound Thioflavin T micelles are observed along the fibril length for representative fibrils. Increasing concentration of Thioflavin T above the critical micellar concentration shows increased numbers of micelles bound along the length of the amyloid fibrils. Thioflavin T micelles are disrupted at low pH as observed by atomic force microscopy and fluorescence enhancement upon binding of Thioflavin T to amyloid fibrils also reduced by several-fold upon decreasing the pH to below 3.The micelles of Thioflavin T bind amyloid fibrils leading to enhancement of fluorescence emission[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
318.86 |
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Formula |
C17H19ClN2S |
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CAS 号 |
2390-54-7 |
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中文名称 |
硫黄素T |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
4deg;C, sealed storage, away from moisture and light *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light) |
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溶解性数据 |
In Vitro:;
DMSO : 22.5 mg/mL (70.56 mM; Need ultrasonic) H2O : 12.5 mg/mL (39.20 mM; Need ultrasonic) 配制储备液
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参考文献 |
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Kinase Assay [1] |
Thioflavin T (ThT) is prepared by dissolving ~3 mg dry powder in 1 mL water. The solution is filtered through 0.22 μm syringe filters followed by measurement of the concentration by diluting the stock solution in ethanol and using an extinction coefficient of 26,620 M-1 cm-1 at 416 nm. The stock solution is stored at 4 °C covered with foil and used for up to a month to make assay solutions by diluting either in water or desired buffer[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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