Rhod-2 AM;
Rhod-2 AM 是一种荧光线粒体探针 (λex=552 nm, λem=581 nm)。
Rhod-2 AM Chemical Structure
CAS No. : 145037-81-6
规格 | 价格 | 是否有货 | |
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1 mg | ¥5800 | 询问价格 货期 |
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生物活性 |
Rhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm). |
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体外研究 (In Vitro) |
A high concentration of Ca2+ in mitochondria is illustrated by punctate labeling in cells, which is consistent with the location of Ca2+ in the mitochondria following fluorescence staining with Rhod-2 AM (Rhod2-AM) at 6 h p.i.. Inhibition of mitochondrial Ca2+ uptake by ruthenium red (RR) or 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS) in IMR5 cells infected with poliovirus (PV) is also illustrated following fluorescence staining with Rhod-2 AM at 6 h p.i.[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
分子量 |
1123.94 |
Formula |
C52H59BrN4O19 |
CAS 号 |
145037-81-6 |
运输条件 |
Room temperature in continental US; may vary elsewhere. |
储存方式 |
-20deg;C, sealed storage, away from moisture and light *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light) |
参考文献 |
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Cell Assay [1] |
For flow cytofluorometry, cells are harvested, pelleted, and resuspended in ice-cold PBS containing 10 mM glucose, 10% fetal bovine serum (FBS), and 10 μM Rhod-2 AM (Rhod2-AM). Mitochondrial calcium levels are determined by the flow cytofluorometry analysis of aliquots of 4×105 cells. For fluorescence microscopy, IMR5 cells are grown on polylysine-coated (10 μg/mL) slides and stained with 7.5 μM Rhod-2 AM in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS for 2 h before poliovirus (PV) infection. Cells are fixed by incubation for 15 min at 4°C in 4% paraformaldehyde. Cells are washed in PBS, and images are acquired with Zeiss Apotome and Axiovision software[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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