Hoechst 33342(Synonyms: bisBenzimide H 33342 HOE 33342)

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Hoechst 33342;(Synonyms: bisBenzimide H 33342; HOE 33342) 纯度: ge;98.0%

Hoechst染料是DNA蓝色荧光染料，Hoechst 33342是其中一种。

Hoechst 33342amp;;(Synonyms: bisBenzimide H 33342; HOE 33342)

Hoechst 33342 Chemical Structure

CAS No. : 23491-52-3

规格 价格 是否有货 数量
25 mg ￥500 In-stock
50 mg ￥800 In-stock
100 mg ￥1200 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

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Hoechst 33342 相关产品

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生物活性

Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.

IC50 Target

Dye reagent[1]
DNA Stain[1]
体外研究
(In Vitro)

Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

452.55

Formula

C27H28N6O
CAS 号

23491-52-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 10 mg/mL (22.10 mM; Need ultrasonic)

H2O : 1 mg/mL (2.21 mM; ultrasonic and warming and heat to 80°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.2097 mL 11.0485 mL 22.0970 mL
5 mM 0.4419 mL 2.2097 mL 4.4194 mL
10 mM 0.2210 mL 1.1049 mL 2.2097 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.
Cell Assay
[1]

Labeling Nuclear DNA with Hoechst 33342[1] Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+. Step 5, Mount the coverslips. Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.