5(6)-Carboxyfluorescein(Synonyms: 5(6)-羧基荧光素 5(6)-FAM 5-(and-6)-Carboxyfluorescein mixed isomers)

发表于由

5(6)-Carboxyfluorescein;(Synonyms: 5(6)-羧基荧光素; 5(6)-FAM; 5-(and-6)-Carboxyfluorescein mixed isomers) 纯度: 99.00%

5(6)-Carboxyfluorescein (5(6)-FAM) 是一种胺反应性 pH 敏感的绿色荧光标记物。5(6)-Carboxyfluorescein 可用于标记蛋白质、多肽和氨基酸。5(6)-Carboxyfluorescein 可用于体内实验肿瘤位置检测。

5(6)-Carboxyfluoresceinamp;;(Synonyms: 5(6)-羧基荧光素; 5(6)-FAM; 5-(and-6)-Carboxyfluorescein mixed isomers)

5(6)-Carboxyfluorescein Chemical Structure

CAS No. : 72088-94-9

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥500 In-stock
500 mg ￥400 In-stock
1 g ￥500 In-stock
5 g ; 询价 ;
10 g ; 询价 ;

* Please select Quantity before adding items.

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生物活性

5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo[1][2].

体外研究
(In Vitro)

5(6)-Carboxyfluorescein has two main characteristics: it has two wavelengths of maximum absorbance (465 and 490 nm) and its fluorescence emission (maximum, 515 nm) increases as a function of pH in the physiological pH range of 6-7.4[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

5(6)-Carboxyfluorescein (5 mg/kg; i.p.) can be used for in vivo pH mapping of tumor tissue[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CDF mice, bearing lymphoid leukemia P388[2]
Dosage: 5 mg/kg
Administration: Injected intraperitoneally
Result: Could be used for measurement and imaging of tumor tissue.

分子量

752.63

Formula

C42H24O14
CAS 号

72088-94-9
中文名称

5(6)-羧基荧光素
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : ≥ 41 mg/mL (54.48 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3287 mL 6.6434 mL 13.2867 mL
5 mM 0.2657 mL 1.3287 mL 2.6573 mL
10 mM 0.1329 mL 0.6643 mL 1.3287 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Maksim V Kvach, et al. 5(6)-carboxyfluorescein revisited: new protecting group, separation of isomers, and their spectral properties on oligonucleotides. Bioconjug Chem. Sep-Oct 2007;18(5):1691-6.

    [2]. Wen Ma, et al. A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback. Adv Healthc Mater. 2020 Jan;9(1):e1901100.