4-Methylumbelliferyl phosphate(Synonyms: 4-MUP MUP)

4-Methylumbelliferyl phosphate;(Synonyms: 4-MUP; MUP) 纯度: 99.93%

4-Methylumbelliferyl phosphate (4-MUP) 是一种碱性磷酸酶的荧光底物。

4-Methylumbelliferyl phosphateamp;;(Synonyms: 4-MUP;  MUP)

4-Methylumbelliferyl phosphate Chemical Structure

CAS No. : 3368-04-5

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10;mM;*;1 mL in Water ¥660 In-stock
50 mg ¥500 In-stock
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4-Methylumbelliferyl phosphate 相关产品

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  • Bioactive Compound Library Plus

生物活性

4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate of alkaline phosphatases[1].

体外研究
(In Vitro)

4-Methylumbelliferyl phosphate monoester previously described as one of a series of 4-methylumbelliferone conjugates is later found to be the PlP2-pyrophosphate diester and is not hydrolysed by serum phosphomonoesterases . 4-Methylumbelliferyl phosphate has a significant advantage over currently available substrates in that its useful concentration range extends to about 0.1 μM[1].
If the concentration of 4-Methylumbelliferyl phosphate is held constant and the pH is varied, the activity of the enzyme passes through a maximum[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

256.15

Formula

C10H9O6P

CAS 号

3368-04-5

中文名称

4-甲基伞形酮磷酸酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

H2O : 20.83 mg/mL (81.32 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.9040 mL 19.5198 mL 39.0396 mL
5 mM 0.7808 mL 3.9040 mL 7.8079 mL
10 mM 0.3904 mL 1.9520 mL 3.9040 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Fernley HN, et al. Kinetic behaviour of calf-intestinal alkaline phosphatase with 4-methylumbelliferyl phosphate. Biochem J. 1965 Oct;97(1):95-103.