Di-8-ANEPPS

Di-8-ANEPPS; 纯度: 99.46%

Di-8-ANEPPS 是萘基苯乙烯基电压敏感染料,它们的荧光激发和发射光谱会因电压的变化而变化。

Di-8-ANEPPSamp;;

Di-8-ANEPPS Chemical Structure

CAS No. : 157134-53-7

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Di-8-ANEPPS 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .

体外研究
(In Vitro)

A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na+-containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the “center” signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

592.87

Formula

C36H52N2O3S

CAS 号

157134-53-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

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参考文献
  • [1]. Manno C, et al. Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS. J Gen Physiol. 2013 Mar;141(3):371-87.

Cell Assay
[1]

To implement the technique, cells enzymatically isolated from mouse flexor digitorum brevis (FDB) muscle are stained by brief exposure to a saline with Di-8-ANEPPS and then washed in solution[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Manno C, et al. Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS. J Gen Physiol. 2013 Mar;141(3):371-87.