Calcein Blue(Synonyms: 钙黄绿素蓝) | Cytiva思拓凡-Whatman滤纸滤膜滤器

Calcein Blue;(Synonyms: 钙黄绿素蓝) 纯度: 98.66%

Calcein Blue 是一种不渗透膜的荧光染料，是一种含有亚氨基二乙酸结构的香豆素衍生物。Calcein Blue 也是一种金属荧光变色指示剂。

Calcein Blue Chemical Structure

CAS No. : 54375-47-2

规格	价格	是否有货	数量
10;mM;*;1 mL in DMSO	￥550	In-stock
25 mg	￥500	In-stock
50 mg	;	询价	;
100 mg	;	询价	;

* Please select Quantity before adding items.

Calcein Blue 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

Calcein Blue, a membrane-impermeant fluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator^[1]^[2]^[3].

体外研究
(In Vitro)

The fluorescence of Calcein Blue (CB) is quenched by the Fe²⁺ ion in the Calcein Blue-Fe²⁺ complex. When dopamine is added to a solution of the Calcein Blue-Fe²⁺ complex, a dopamine-Fe²⁺ complex is formed as the result of a ligand exchange reaction between Calcein Blue and dopamine which permits the fluorescence from Calcein Blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the Calcein Blue-Fe²⁺ complex solution, which permits dopamine to be quantitatively determined. The Calcein Blue-Fe²⁺ complex shows a high selectivity for dopamine among the catecholamines and related compounds examined^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

321.28

Formula

C₁₅H₁₅NO₇

CAS 号

54375-47-2

中文名称

钙黄绿素蓝

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据

In Vitro:;

DMSO : 6 mg/mL (18.68 mM; Need ultrasonic and warming)

H₂O : 1 mg/mL (3.11 mM; ultrasonic and warming and heat to 80°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.1125 mL	15.5627 mL	31.1255 mL
5 mM	0.6225 mL	3.1125 mL	6.2251 mL
10 mM	0.3113 mL	1.5563 mL	3.1125 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

参考文献

[1]. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30;94:36-43.

[2]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49.

[3]. G M Huitink, et al. Methyl calcein blue and other analogues of calcein blue. Talanta. 1974 Nov;21(11):1193-202.

Cell Assay	The recommended final working concentrations is usually between 1 and 10 μM to minimize potential artifacts. Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processing of the dyes. A stock concentration of 5 mM 4-methylumbelliferone-8- methyliminodiacetic acid, commonly known as calcein blue is prepared in 0.1 M potassium hydroxide, and the pH is neutralized using 0.1 N hydrochloric acid. The calcein blue stock prepared is diluted to 200 μM (working stock) in DPBS and stored for further use. A concentration of 10 μM calcein blue is prepared from the working stock by diluting in DPBS. From this, 200 μL is added to a 96-well plate and the excitation/emission (Ex./Em.) maximum is determined using the micro-plate reader^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30;94:36-43. [2]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49. [3]. G M Huitink, et al. Methyl calcein blue and other analogues of calcein blue. Talanta. 1974 Nov;21(11):1193-202.

Cell Assay

The recommended final working concentrations is usually between 1 and 10 μM to minimize potential artifacts. Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processing of the dyes. A stock concentration of 5 mM 4-methylumbelliferone-8- methyliminodiacetic acid, commonly known as calcein blue is prepared in 0.1 M potassium hydroxide, and the pH is neutralized using 0.1 N hydrochloric acid. The calcein blue stock prepared is diluted to 200 μM (working stock) in DPBS and stored for further use. A concentration of 10 μM calcein blue is prepared from the working stock by diluting in DPBS. From this, 200 μL is added to a 96-well plate and the excitation/emission (Ex./Em.) maximum is determined using the micro-plate reader^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30;94:36-43.

[2]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49.

[3]. G M Huitink, et al. Methyl calcein blue and other analogues of calcein blue. Talanta. 1974 Nov;21(11):1193-202.