Arg-Gly-Asp-Cys | Cytiva思拓凡-Whatman滤纸滤膜滤器

Arg-Gly-Asp-Cys;

Arg-Gly-Asp-Cys 是纤维粘连蛋白与细胞粘附分子的结合基序，能够抑制血小板凝集以及纤维蛋白原结合。

Arg-Gly-Asp-Cys Chemical Structure

CAS No. : 109292-46-8

规格	价格	是否有货
5 mg	￥770	询问价格货期
10 mg	￥1250	询问价格货期
25 mg	￥2500	询问价格货期

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Arg-Gly-Asp-Cys 的其他形式现货产品:

Arg-Gly-Asp-Cys TFA

生物活性

Arg-Gly-Asp-Cys is the binding motif of fibronectin to cell adhesion molecules, and can inhibit platelet aggregation and fibrinogen binding.

体外研究
(In Vitro)

RGDC immobilizes peptide onto DAH-CMTMC is found to be about 15.3 μg/mg of chitosan derivative by amino acid analysis (AAA). RGDC-functionalized chitosan may lead to enhanced wound healing (viability >140%). RGDC-functionalizes chitosan derivatives exhibit in vitro wound healing properties by enhancing fibroblast proliferation and adhesion. RGDC-DAH-CMTMC favors cell growth and an increase in cellular proliferation compared to the control cells^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

449.48

Formula

C₁₅H₂₇N₇O₇S

CAS 号

109292-46-8

Sequence Shortening

RGDC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-80deg;C	2 years
	-20deg;C	1 year
In solvent	-80deg;C	6 months
	-20deg;C	1 month

Solvent Solubility

In Vitro:;

H₂O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

;	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3.;;Recommended solution:

Overall charge of peptide	Details
Negative (lt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH₄OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

参考文献

[1]. Patrulea V, et al. Peptide-decorated chitosan derivatives enhance fibroblast adhesion and proliferation in wound healing. Carbohydr Polym. 2016 May 20;142:114-23.

Cell Assay ^[1]	Human precursor dermal fibroblasts (HDF, human dermal progenitor cells, 12 week male donor) are use in the assay. WST-1 assay is used to assess the viability of HDF when incubated with chitosan derivatives. For this study, HDF are seeded in a 96-well plate at a density of 6×10³ cells/cm². To each well, 100 μL of cell suspension is added and incubated for 48 h in order to allow cell attachment. DMEM is then replaced by 100 μL of CMTMC and RGDC-DAH-CMTMC suspension at concentrations of 0.25 mg/mL, 0.5 mg/mL and 1 mg/mL, respectively. Cell viability under polymer incubation is evaluated during 2, 4 and 7 days. SDS (1%) is used as negative control. The polymer solution is changed every 3 days. 100 μL of WST-1 (1:10 dilution in DMEM) are added in each well after removing the polymer suspension and incubated for 0.5-2 h. Absorbance is recorded with a BioTek Microplate reader at two different wavelengths (450 and 690 nm). The viability is presented as percentage compared to the positive control group (cells in DMEM supplemented with 10% fetal calf serum). All experiments are carried out in triplicates. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Patrulea V, et al. Peptide-decorated chitosan derivatives enhance fibroblast adhesion and proliferation in wound healing. Carbohydr Polym. 2016 May 20;142:114-23.

Cell Assay
^[1]

Human precursor dermal fibroblasts (HDF, human dermal progenitor cells, 12 week male donor) are use in the assay. WST-1 assay is used to assess the viability of HDF when incubated with chitosan derivatives. For this study, HDF are seeded in a 96-well plate at a density of 6×10³ cells/cm². To each well, 100 μL of cell suspension is added and incubated for 48 h in order to allow cell attachment. DMEM is then replaced by 100 μL of CMTMC and RGDC-DAH-CMTMC suspension at concentrations of 0.25 mg/mL, 0.5 mg/mL and 1 mg/mL, respectively. Cell viability under polymer incubation is evaluated during 2, 4 and 7 days. SDS (1%) is used as negative control. The polymer solution is changed every 3 days. 100 μL of WST-1 (1:10 dilution in DMEM) are added in each well after removing the polymer suspension and incubated for 0.5-2 h. Absorbance is recorded with a BioTek Microplate reader at two different wavelengths (450 and 690 nm). The viability is presented as percentage compared to the positive control group (cells in DMEM supplemented with 10% fetal calf serum). All experiments are carried out in triplicates.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Patrulea V, et al. Peptide-decorated chitosan derivatives enhance fibroblast adhesion and proliferation in wound healing. Carbohydr Polym. 2016 May 20;142:114-23.