3x DYKDDDDK Tag;
3x DYKDDDDK Tag 是研究抗体 (Abs) 不可用的蛋白质的功能和定位的有用工具。通常,它也以 3X 标志形式 (3x DYKDDDDK Tag TFA) 用于纯化低丰度蛋白。
3x DYKDDDDK Tag Chemical Structure
CAS No. : 921765-76-6
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生物活性 |
3x DYKDDDDK Tag is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance [1][2]. |
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体外研究 (In Vitro) |
HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
分子量 |
3002.88 |
Formula |
D15K6Y3 |
CAS 号 |
921765-76-6 |
Sequence Shortening |
DYKDDDDKDYKDDDDKDYKDDDDK |
运输条件 |
Room temperature in continental US; may vary elsewhere. |
储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis. |
参考文献 |
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