Gliadin p31-43

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Gliadin p31-43;

Gliadin p31-43 是一种未消化的麦醇溶蛋白肽，可诱导肠道内的先天性免疫反应并干扰内吞运输。Gliadin p31-43 可用于乳糜泻的研究。

Gliadin p31-43amp;;

Gliadin p31-43 Chemical Structure

CAS No. : 176326-01-5

规格 价格 是否有货 数量
5 mg ￥5500 In-stock
10 mg ￥8800 In-stock
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100 mg ; 询价 ;

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Gliadin p31-43 相关产品

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  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Gliadin p31-43 is an undigested gliadin peptide. Gliadin p31-43 induces an innate immune response in the intestine and interferes with endocytic trafficking. Gliadin p31-43 can be used for celiac disease research[1][2].

体外研究
(In Vitro)

Gliadin p31-43 (100 μg/mL; 30 minutes-6 hours) treatment induces the MyD88/TLR7 complexes, and activates downstream signalling by activating MAPKs, ERK, JNK and p38). Gliadin p31-43 increases the levels of the phosphorylated forms of pY-ERK, JNK (pY-JNK) and p38 (pY-p38)[1].
Gliadin p31-43 treatment increases NF-κB phosphorylation in CaCo-2 cells from 0.45 in control cells to 0.86. Gliadin p31-43 treatment induces a significant increase in levels of the MxA protein. The levels of the IFN-α 7 and 17 mRNAs are also analysed after Gliadin p31-43 treatment[1].
In CaCo-2 cells, Gliadin p31-43 localizes to the early endosomes and delays vesicular trafficking. Gliadin p31-43 interferes with the correct localization of the growth factor regulated tyrosine kinase substrate (HRS) to early endosomes, delaying the maturation of the endocytic vesicles[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: CaCo-2 cells
Concentration: 100 μg/mL
Incubation Time: 30 minutes, 3 hours, 6 hours
Result: Showed the increase in formation of the MyD88/TLR7 complex, and increased in the level of TLR7.

体内研究
(In Vivo)

Gliadin p31-43 (10 μg; intraluminally injection) shows a sequence-specific spontaneous ability to form structured oligomers and aggregates in vitro and induced activation of the apoptosis-associated speck-like (ASC) complex[2].
The increment of IL-1β indicates the activation of the inflammasome caspase-1 pathway in the small intestine mucosa by oral administration of Gliadin p31-43 (20 μg) in wild type C57Bl/6 mice. Gliadin p31-43 has an intrinsic propensity to form oligomers which trigger the NLRP3 inflammasome[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1527.68

Formula

C71H102N18O20
CAS 号

176326-01-5
Sequence Shortening

LGQQQPFPPQQPY
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 50 mg/mL (32.73 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.6546 mL 3.2729 mL 6.5459 mL
5 mM 0.1309 mL 0.6546 mL 1.3092 mL
10 mM 0.0655 mL 0.3273 mL 0.6546 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Merlin Nanayakkara, et al. P31-43, an undigested gliadin peptide, mimics and enhances the innate immune response to viruses and interferes with endocytic trafficking: a role in celiac disease. Sci Rep. 2018 Jul 17;8(1):10821.

    [2]. María Florencia Gómez Castro, et al. p31-43 Gliadin Peptide Forms Oligomers and Induces NLRP3 Inflammasome/Caspase 1- Dependent Mucosal Damage in Small Intestine. Front Immunol. 2019 Jan 30;10:31.