S Tag Peptide(Synonyms: S标签肽)

发表于由

S Tag Peptide;(Synonyms: S标签肽)

S Tag Peptide 是一种合成的多肽，由15个氨基酸残基构成。

S Tag Peptideamp;;(Synonyms: S标签肽)

S Tag Peptide Chemical Structure

规格 价格 是否有货
1 mg ￥500 询问价格 货期
5 mg ￥1100 询问价格 货期
10 mg ￥1900 询问价格 货期

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生物活性

S Tag Peptide is a 15 amino acid peptide derived from RNase A.

体外研究
(In Vitro)

S Tag sequence is a novel fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. S Tag can be applied to purification of target proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1748.91

Formula

C73H117N23O25S
Sequence

Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser
Sequence Shortening

KETAAAKFERQHMDS
中文名称

S标签肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Mark McCormick, et al. S Tag: A Multipurpose Fusion Peptide for Recombinant Proteins.