Gap 27; 纯度: 98.07%
Gap 27，一种合成的 connexin43 模拟肽，是一种间隙连接抑制剂。Gap 27 与通往第二跨膜连接蛋白区段的第二细胞外环的一部分具有保守的序列同源性。
Gap 27 Chemical Structure
CAS No. : 198284-64-9
| 规格 | 价格 | 是否有货 | 数量 |
|---|---|---|---|
| 1 mg | ￥700 | In-stock | |
| 5 mg | ￥1300 | In-stock | |
| 10 mg | ; | 询价 | ; |
| 50 mg | ; | 询价 | ; |
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Gap 27 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
- Peptide Library
| 生物活性 |
Gap 27, a synthetic connexin43 mimetic peptide, is a gap junction inhibitor. Gap 27 possesses conserved sequence homology to a portion of the second extracellular loop leading into the fourth transmembrane connexin segment[1][2]. |
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| 体外研究 (In Vitro) |
Gap 27 causes a remarked decrease in the number of both TRAP-positive mononuclear and multinucleated rat osteoclasts cultured on bovine bone slices. The activity of the remaining osteoclasts is found to be diminished by measuring the percentage of osteoclasts with actin rings of all TRAP-positive cells. In addition, the resorbed area in the treated cultures is greatly diminished[1]. Incubation of the carotid artery with the gap junction inhibitor Gap 27 (500 μM) essentially abolishes the hyperpolarization to acetylcholine but it is without effect on that to levcromakalim. In the guinea-pig isolated internal carotid artery, Gap 27 inhibits acetylcholine-induced, endothelium-dependent hyperpolarizations[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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| 体内研究 (In Vivo) |
Gap 27 (300 μM) inhibits relaxation by 40% in thoracic aorta and the superior mesenteric artery. Gap 27 also attenuates the endothelium-dependent component of the relaxation induced by ATP in thoracic aorta. [3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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| 分子量 |
1304.53 |
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| Formula |
C60H101N15O17 |
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| CAS 号 |
198284-64-9 |
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| Sequence |
Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile |
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| Sequence Shortening |
SRPTEKTIFII |
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| 运输条件 |
Room temperature in continental US; may vary elsewhere. |
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| 储存方式 |
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| 溶解性数据 |
In Vitro:;
H2O : 33.33 mg/mL (25.55 mM; Need ultrasonic) 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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| 参考文献 |
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| Cell Assay [1] |
Bone cell cultures are cultured for 48 hours with three different treatments (control, heptanol and Gap 27). After the culture period, bone slices are fixed. The cells are stained for tartrate-resistant acid phosphatase (TRAP). To visualise the nuclei, the cells are incubated with the DNA-binding fluorochrome Hoechst 33258 (1 mg/mL stock diluted 1:800 in PBS) for 10 minutes at room temperature. The numbers of mononuclear and multinucleated TRAP-positive cells on each bone slice are counted[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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| 参考文献 |
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