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Products > Optimize Reagents > Optimize – Buffers > ADA Buffer

ADA Buffer

应用

  • Crystallization grade ADA buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 ADA 缓冲液

特征

  • Sterile filtered 无菌过滤
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

ADA (Buffer)

Synonyms: N-(2-Acetamido)iminodiacetic acid;
N-(Carbamoylmethyl)iminodiacetic acid
C6H10N2O5
H2NCOCH2N(CH2CO2H)2
Mr 190.16
CAS Number [26239-55-4]
EC Number 247-530-0
Beilstein Registry Number 1787181
Merck 14,147
Purity ≥ 99.0%
MDL Number MFCD00008031
PubChem Substance ID 24845005
pKa = 6.6 at 25°Celsius

Useful pH range of ADA is 5.6 – 7.5

Titrate HR2-507 to the desired pH using NaOH
HR2-817 is titrated to pH 6.5 using NaOH

HR2-507 measured Conductivity range: 23.6 – 28.6 mS/cm at 25°C
HR2-507 measured Refractive Index range: 1.35009 – 1.35044 at 20°C

HR2-817 measured Conductivity range: 42.2 – 50.4 mS/cm at 25°C
HR2-817 measured Refractive Index range: 1.36787 – 1.36913 at 20°C

ADA buffer is used in the Hampton Research MembFac crystallization kit.ADA 缓冲液用于 Hampton Research MembFac 结晶试剂盒。

Total Impurities Insoluble matter, passes filter test总杂质 不溶物,通过过滤测试
Al  ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.1%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.05%
Ni  ≤0.0005%
Pb  ≤0.0005%
SO4 ≤0.005%
Sr  ≤0.0005%
Zn  ≤0.0005%

Hampton蛋白结晶试剂盒ADA Buffer

CAT NO

HR2-507

NAME

0.5 M ADA

DESCRIPTION

100 mL

PRICE

$123.00

CAT NO

HR2-817

NAME

1.0 M ADA pH 6.5

DESCRIPTION

100 mL

PRICE

$132.00

SDS

相关项目

  • Sodium hydroxide 氢氧化钠
  • Individual StockOptions ADA Reagents 个人股票期权 ADA 试剂
  • StockOptions ADA Buffer Kit StockOptions ADA 缓冲套件

参考

1. Structures of the noncovalent complexes of human and bovine prothrombin fragment 2 with human PPACK-thrombin. Arni, RK; Padmanabhan, K; Padmanabhan, KP; Wu, TP; Tulinsky, A. Biochemistry 32, 4727- 4737, 1993.

2. Structures of Three Crystals Forms of the Sweet Protein Thaumatin. Ko, TP; Day, J; Greenwood, A; McPherson, A. Acta Crystallogr D 50, 813- 825, 1994.

1.人和牛凝血酶原片段2与人PPACK-凝血酶的非共价复合物的结构。 阿尼,RK; 帕德马纳班,K; 帕德马纳班,KP; 吴,TP; Tulinsky, A. Biochemistry 32, 4727-4737, 1993。

2. 甜蛋白奇异果甜蛋白的三种晶型结构。 高,TP; 日,J; 格林伍德,一个; McPherson, A. 晶体学学报 D 50, 813-825, 1994。