StemAb™ 干细胞用碱性磷酸酶染色试剂盒
- 产品特性
- 相关资料
- Q&A
- 参考文献
StemAb™ 干细胞用碱性磷酸酶染色试剂盒
碱性磷酸酶(AP)在多能干细胞中高水平表达。 AP 在碱性条件下使核苷酸、蛋白和生物碱去磷酸化。 染色后,未分化细胞呈现红色或紫色,而分化细胞呈现无色。
胚胎干细胞(ES)和诱导多能干细胞(iPS)的未分化状态可以通过高水平的碱性磷酸酶(AP)表达来表征,连同其他表面标志物的表达,表明未分化细胞具有自我更新潜力。AP 是一种水解酶,负责在碱性条件下使核苷酸,蛋白和生物碱去磷酸化。 当使用 AP 染色试剂盒对固定的 ES 或 iPS 细胞染色时,未分化细胞呈现红色或紫色,而分化细胞呈现无色。
◆试剂盒组成
固定溶液: 25 mL
AP 染色溶液 A:10 mL
AP 染色溶液 B:10 mL
AP 染色溶液 C:10 mL
◆储存和稳定性
● 在 4°C 储存, 不可冷冻储存。到货后妥善存储可稳定 6 个月。
◆质量控制
StemAb™ 干细胞用碱性磷酸酶染色试剂盒已在人 iPS 细胞和小鼠 ES 细胞上进行功能测试,确保产品质量。
◆操作步骤
StemAb Alkaline Phosphatase Staining Kit II
※ 本页面产品仅供研究用,研究以外不可使用。
参考文献
1. |
Pease, S., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. (1990) Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 141: 344-352.全文 |
2. |
Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA/LIF) and mouse development. Dev. Biol. 151: 339-351. 全文 |
3. |
Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872. 全文 |
4. |
Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.全文 |
◆其他相关资料
1. |
Méjécase C; Harding P; Sarkar J; Eintracht J; Cunha DL; Toualbi L; Moosajee M. Generation of two human control iPS cell lines (UCLi016-A and UCLi017-A) from healthy donors with no known ocular conditions. Stem Cell Res 49:102113 (2020).全文 |
2. |
Yoo J; La Y; Lee EJ; Choi J-J; Oh J; Thang NX; Hong K. ATP-Dependent chromatin remodeler CHD9 controls the proliferation of embryonic stem cells in a cell culture condition-dependent manner. Biology 9:428 (2020). 全文 |
3. |
Chung CY; Lo PHY; Lee KKH. Babam2 Regulates Cell Cycle Progression and Pluripotency in Mouse Embryonic Stem Cells as Revealed by Induced DNA Damage. Biomedicines 8:397 (2020).全文 |
4. |
Torzal F; Lau QY; Ibuki M; Kawai T; Horikawa M; Minami M; Horiguchi I; Nishikawa M; Sakai Y. High-density hiPSCs expansion supported by growth factors accumulation in a simple dialysis-culture platform. Research Square ://doi.org/10.21203/rs.3.rs-71643/v1(2020). 全文 |
5. |
Choi NY; Bang JS; Park YS; Lee M; Hwange HS; Ko K; Myung SC; Tapia N; Schöler HR; Kim GJ; Ko K. Generation of human androgenetic induced pluripotent stem cells. Sci Rep 10:3614 (2020).全文 |
6. |
Hu X; Wu Q; Zhang J; Chen X; Hartman A; Eastman A: Guo S. Reprogramming progressive cells display low CAG promoter activity. bioRxiv :https://doi.org/10.1101/2020.03.03.975664 (2020).全文 |
7. |
Parfenov VA; Mironov VA; Koudan EV; Nezhurina EK; Karalkin PA; Pereira FDAS; Petrov SV; Krokhmal KK; Tydemir R; Vakhrushev EV; Zobkov YV; Smirnov IV; Fedotov AY; Demirci U; Khesuani YD; Komlev VS. Fabrication of calcium phosphate 3D scaffolds for bone repair using magnetic levitational assembly. Sci Rep 10:4013 (2020).全文 |
8. |
Choi NY; Bang JS; Park YS; Lee M; Hwange HS; Ko K; Myung SC; Tapia N; Schöler HR; Kim GJ; Ko K. Generation of human androgenetic induced pluripotent stem cells. Sci Rep 10:3614 (2020). 全文 |
9. |
Ha M; Athirasala A; Tahayeri A; Menezes PP; Bertassoni LE. Micropatterned hydrogels and cell alignment enhance the odontogenic potential of stem cells from apical papilla in-vitro. Dental Materials in press:doi.org/10.1016/j.dental.2019.10.013 (2019).全文 |
10. |
Hao Y; Fan X; Shi Y; ZXhang C; Sun E-E; Qin K; Qin W; Zhou W; Chen X. Next-generation unnatural monosaccharides reveal that ESRRB O-GlcNAcylation regulates pluripotency of mouse embryonic stem cells. Nature Commun 10:4065 (2019). 全文 |
11. |
Ge N; Liu M; Krawczyz J; McInerney V; Galvin J; Shen S; O'Brien T; Prendiville T. Generation of eight human induced pluripotent stem cell (iPSC) lines from familial Long QT Syndrome type 1 (LQT1) patients carrying KCNQ1 c.1697C>A mutation (NUIGi005-A, NUIGi005-B, NUIGi005-C, NUIGi006-A, NUIGi006-B, NUIGi006-C, NUIGi007-A, and NUIGi007-B). Stem Cell Res in press:https://doi.org/10.1016/j.scr.2019.101502 (2019).全文 |
12. |
Mura M; Pisano F; Stefanelllo M; Cinevrino M; Boni M; Calabrò F; Crotti L; Valente EM; Schwartz PJ; Brink PA; Gnecchi M. Generation of two human induced pluripotent stem cell (hiPSC) lines from a long QT syndrome South African founder population. Stem Cell Res in Press:https://doi.org/10.1016/j.scr.2019.101510 (2019). 全文 |
13. |
Headley KM; Kedziora KM; Alejo A; Lai EZ-X; Purvis JE; Hathaway NA. Chemical screen for epigenetic barriers to single allele activation of Oct4. Stem Cell Res in Press:https://doi.org/10.1016/j.scr.2019.101470 (2019).全文 |
14. |
Johari B; Asadi Z; Rismani E; Maghsood F; Rezaei ZS; Farahani S; Madanchi H; Kadivar M. Inhibition of transcription factor T‐cell‐factor 3 (TCF3) using oligodeoxynucleotide strategy increases embryonic stem cell stemness: The possible application in regenerative medicine. Cell Biol Intl in press:doi: 10.1002/cbin.11153 (2019). 全文 |
15. |
Watanabe T; Yamazaki S; Yoneda N; Shinohara H; Tomioka I; Iiguchi T; Tagoto M; Ema M; Suemizu H; Kawai K; Sasaki E. Highly efficient induction of primate iPS cells by combining RNA transfection and chemical compounds. Genes to Cells in press:doi:10.1111/gtc.12702 (2019).全文 |
16. |
Panina Y. Development of a new high-precision quantification methodology for gene expression analysis and its application in iPS cells. Doctoral Thesis, Osaka University : (2019).全文 |
17. |
Soda M; Saitoh I; Murakami T; Inada E; Iwase Y; Noguchi H; Shibasaki S; Murosawa M; Sawami T; Terunuma M; Kubota N; Terao Y; Ohshima H; Hayasaki H; Sato M. Repeated human deciduous tooth-derived dental pulp cell reprogramming factor transfection yields multipotent intermediate cells with enhanced iPS cell formation capability. Sci Rep 9:1490 (2019). 全文 |
18. |
Lin VJT; Zolekar A; Shi Y; Koneru B; Dimitrijevich S; Di Pasqua AJ; Wang C-Y. Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells. Sci Reports 7:5005 (2017). 全文 |
19. |
Momcilovic O; Sivapatham R; Oron TR; Meyer M; Mooney S; Rao MA; Zeng X. Derivation, characterization, and neural differentiation of integration-free induced pluripotent stem cell lines from Parkinson's disease patients carrying SNCA, LRRK2, PARK2, and GBA mutations. _PLoS ONE_ 11(5): e0154890. doi:10.1371/journal.pone.0154890 (2016). 全文 |
20. |
Borenstrom C; Simonsson S; Enochson L; BIgdeli N; Branstsing C; Ellerstrom C; Hyllner J; Lindahl A. Footprint-Free Human Induced Pluripotent Stem Cells From Articular Cartilage With Redifferentiation Capacity: A First Step Toward a Clinical-Grade Cell Source. Stem Cells Translat Med 3:433-447 (2014). 全文 |
产品编号 | 产品名称 | 产品规格 | 产品等级 | 备注 |
00-0055 | Stemgent Alkaline Phosphatase Staining Kit II (50 assays) Stemgent碱性磷酸酶染色试剂盒II |
50 assays | - | - |