DMA | Cytiva思拓凡-Whatman滤纸滤膜滤器

DMA; 纯度: 99.64%

DMA 是一种荧光化合物 (λ_ex=340 nm, λ_em=478 nm)。

DMA Chemical Structure

CAS No. : 188860-26-6

规格	价格	是否有货	数量
10;mM;*;1 mL in DMSO	￥660	In-stock
5 mg	￥600	In-stock
10 mg	￥900	In-stock
50 mg	￥3600	In-stock
100 mg	￥6600	询价
200 mg	;	询价	;
500 mg	;	询价	;

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DMA 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus
Anti-Cancer Compound Library

生物活性

DMA is a fluorescent compound (λ_ex=340 nm, λ_em=478 nm).

IC₅₀ Target

IC50: 3.4 μM (HeLa cell), 5.3 μM (MCF7 cell)^[1]

体外研究
(In Vitro)

The newly synthesized bisbenzimidazole derivatives DMA (6c) is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), breast carcinoma cell line (MCF7) and brain glioma cell line (U87) in comparison to Hoechst. In case of MCF7, the IC₅₀ is observed at 5.3 μM for DMA. The IC₅₀ determined in the case of HeLa is 3.4 μM for DMA^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

468.55

Formula

C₂₇H₂₈N₆O₂

CAS 号

188860-26-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20deg;C	3 years
	4deg;C	2 years
In solvent	-80deg;C	6 months
	-20deg;C	1 month

溶解性数据

In Vitro:;

DMSO : ≥ 54 mg/mL (115.25 mM)

* “≥” means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.1342 mL	10.6712 mL	21.3424 mL
5 mM	0.4268 mL	2.1342 mL	4.2685 mL
10 mM	0.2134 mL	1.0671 mL	2.1342 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

参考文献

[1]. Singh M, et al. Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-aryl-substituted 2-bis-1H-benzimidazoles. Eur J Med Chem. 2011 Feb;46(2):659-69.

Cell Assay	Various human tumor cells (U87, HeLa and MCF7) are maintained as monolayer at 37°C in 5% CO₂ using DMEM medium. Approximately 3000-8000 cells/well are seeded in 96-well plates containing 200 μL of medium and incubated for 24 h. The culture medium is replaced by fresh medium containing 1, 10, 50, 100 μM of DMA (6c) and incubated for 24, 48 and 72 h. The cell viability is determined by the MTT assay. The light absorbance is measured using a microplate reader^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Singh M, et al. Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-aryl-substituted 2-bis-1H-benzimidazoles. Eur J Med Chem. 2011 Feb;46(2):659-69.

Cell Assay

Various human tumor cells (U87, HeLa and MCF7) are maintained as monolayer at 37°C in 5% CO₂ using DMEM medium. Approximately 3000-8000 cells/well are seeded in 96-well plates containing 200 μL of medium and incubated for 24 h. The culture medium is replaced by fresh medium containing 1, 10, 50, 100 μM of DMA (6c) and incubated for 24, 48 and 72 h. The cell viability is determined by the MTT assay. The light absorbance is measured using a microplate reader^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Singh M, et al. Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-aryl-substituted 2-bis-1H-benzimidazoles. Eur J Med Chem. 2011 Feb;46(2):659-69.