Bromobimane;(Synonyms: Monobromobimane) 纯度: 99.06%
Bromobimane基本上是非荧光物质,当与小硫醇反应时,生成荧光产物。
Bromobimane Chemical Structure
CAS No. : 71418-44-5
规格 | 价格 | 是否有货 | 数量 |
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10;mM;*;1 mL in DMSO | ¥660 | In-stock | |
10 mg | ¥600 | In-stock | |
25 mg | ¥1100 | In-stock | |
50 mg | ; | 询价 | ; |
100 mg | ; | 询价 | ; |
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Bromobimane 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
生物活性 |
Bromobimane is essentially nonfluorescent and converts into fluorescent products when reacts with small thiols. |
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体外研究 (In Vitro) |
Bromobimanes in solution (aqueous or organic solvents of medium polarity) react with small thiols [e.g., the tripeptide thiol glutathione (GSH)], and with reactive protein thiol groups (e.g., hemoglobin). The reactions of bromobimanes with thiols are second order and dependent on pH, the active nucleophile being the thiolate anion. The reaction of bromobimane with a thiolate converts the nonfluorescent agent into water-soluble fluorescent products. The neutral agents mBBr and bBBr are moderately soluble in mediumpolarity organic solvents (acetonitrile, dichloromethane), and slightly soluble in water. The quaternary salt, qBBr, and the anionic bromobimane, SBBr, are soluble in water, but less soluble in organic solvents. Bromobimanes are yellow[1]. The highly selective, rapid reactivity of bromobimanes toward thiols, the stability and fluorescence yield of the thiol derivatives, the ease of separation of the derivatives by reversed-phase HPLC, and the availability of both cell-penetrating and nonpenetrating forms, make the use bromobimanes an extremely powerful approach to the analysis of low molecular weight biothiols[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
271.11 |
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Formula |
C10H11BrN2O2 |
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CAS 号 |
71418-44-5 |
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中文名称 |
溴二胺;单溴二胺 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
4deg;C, protect from light *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light) |
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溶解性数据 |
In Vitro:;
DMSO : ≥ 86.6 mg/mL (319.43 mM) * “≥” means soluble, but saturation unknown. 配制储备液
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
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参考文献 |
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Cell Assay [2] |
The stock mBBr solution is prepared by weight at 100-180 mM in acetonitrile. The stock solution of SBBr is prepared by weight at 50 mM in dimethyl sulfoxide (DMSO) or at 2 mM in the 20 mM Tris-methane sulfonate. The thiol is added to a final concentration of 1 mM to a solution of 2 mM mBBr or SBBr, 20 mM Tris-methane sulfonate, pH 8.0. The mixture is incubated in the dark for 15 min at room temperature and then methanesulfonic acid is added to 25 mM from a 5 M stock solution[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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