Fluorescein di(β-D-galactopyranoside);(Synonyms: FDG)
Fluorescein di(β-D-galactopyranoside) 是 β-半乳糖苷酶的荧光底物 (λex=485 nm,λem=535 nm)。
Fluorescein di(β-D-galactopyranoside) Chemical Structure
CAS No. : 17817-20-8
规格 | 价格 | 是否有货 | |
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1 mg | ¥1500 | 询问价格 货期 |
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生物活性 |
Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm). |
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体外研究 (In Vitro) |
The fluorescence produced by Fluorescein di(β-D-galactopyranoside) increases in a time- and dose-dependent manner. The level of fluorescence produced by the double-substrate method is much lower than that by the Fluorescein di(β-D-galactopyranoside) method. Results show that the fluorescein produced by Fluorescein di(β-D-galactopyranoside) in Hs68 cells is proportional to the number of passages[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
分子量 |
656.59 |
Formula |
C32H32O15 |
CAS 号 |
17817-20-8 |
中文名称 |
荧光素二(β-D-吡喃半乳糖苷) |
运输条件 |
Room temperature in continental US; may vary elsewhere. |
储存方式 |
-20deg;C, protect from light *该产品在溶液状态不稳定,建议您现用现配,即刻使用。 |
参考文献 |
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Cell Assay [1] |
The cells (5×103 cells per well) are cultured in a 96-well plate overnight for attachment, washed, and then fixed in solutions. An aliquot (100 μL) of the reaction buffer (i.e., the staining solution without X-Gal) is added into each well. Then, 10 μL of 2 mM Fluorescein di(β-D-galactopyranoside) is added per well and the plate is incubated in the dark at 37°C for 24 h without CO2 supply. After incubation at 37°C for 24 h, 100 μL of the supernatant is transferred to a 96-well plate for fluorescent measurement in triplicates. The fluorescein fluorescence is measured using a fluorometer with an excitation at 485 nm and an emission at 535 nm[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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