QL9; 纯度: 98.49%
QL9 是针对2C T细胞受体 (TCR) 的高亲和力同种异体抗原。
QL9 Chemical Structure
CAS No. : 159646-83-0
规格 | 价格 | 是否有货 | 数量 |
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1 mg | ¥1200 | In-stock | |
5 mg | ¥4800 | In-stock | |
10 mg | ¥8100 | In-stock | |
50 mg | ; | 询价 | ; |
100 mg | ; | 询价 | ; |
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QL9 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
- Peptide Library
生物活性 |
QL9 (QLSPFPFDL) is a high-affinity alloantigen for the 2C T cell receptor (TCR). |
IC50 Target |
TCR[1] |
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体外研究 (In Vitro) |
Mouse T cell clone 2C recognizes two different major histocompatibility (MHC) ligands, the self MHC Kb and the allogeneic MHC Ld. Two distinct peptides, SIY (SIYRYYGL) and QL9 (QLSPFPFDL), act as strong and specific agonists when bind to Kb and Ld, respectively. QL9 binding to MHC Ld is influenced by the majority of peptide side chains, distributed across the entire length of the peptide. Findings with both systems, but QL9-Ld in particular, suggest that many single-residue substitutions, introduced into peptides to improve their binding to MHC and thus their vaccine potential, could impair T cell reactivity due to their dual impact on TCR binding. T cell activation assays are performed to measure effects of peptide SIY and QL9 residues on T cell function[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
1063.21 |
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Formula |
C52H74N10O14 |
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CAS 号 |
159646-83-0 |
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Sequence |
Gln-Leu-Ser-Pro-Phe-Pro-Phe-Asp-Leu |
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Sequence Shortening |
QLSPFPFDL |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
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溶解性数据 |
In Vitro:;
H2O : 33.33 mg/mL (31.35 mM; Need ultrasonic) 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
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参考文献 |
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Cell Assay [2] |
Wild type 2C and high affinity 2C T cell transfectants m67 and m6 are incubated with Kb– or Ld-positive cells and various concentrations of peptide SIY and QL9 alanine variants. T cell activation is measured by assaying for levels of IL-2 release. Briefly, T cell transfectants (7.5×104) are incubated with T2-Kb (7.5×104) or T2-Ld (7.5×104) along with various concentrations of peptide for 20-24 h at 37 °C and 5% CO2. Supernatant is harvested, and levels of IL-2 are measured in an enzyme-linked immunosorbent assay type format. Results are plotted as percentage of maximal IL-2 release=((A450 (sample)-A450(no peptide))/(Max A450(sample)-A450(no peptide)))×100; signal obtained from no peptide is similar to that obtained for the null peptides MCMV or OVA. Binding curves are generated in GraphPad Prism by plotting the percentage of maximal IL-2 release against peptide concentration. The concentrations of peptide yielding 50% maximal IL-2 release (SD50) are calculated using non-linear regression (sigmoidal fitting; GraphPad Prism) of the activation curves[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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