Abz-FRLKGGAPIKGV-EDDNP TFA

Abz-FRLKGGAPIKGV-EDDNP TFA;

Abz-FRLKGGAPIKGV-EDDNP TFA 是一种荧光底物,可测量蛋白酶的酶促活性,例如严重急性呼吸系统综合症冠状病毒 (SARS-CoV) 中的木瓜蛋白酶样蛋白酶 2 (PLP2)。Abz-FRLKGGAPIKGV-EDDNP TFA 具有用于研究 2019-nCoV (COVID-19) 感染的潜力。

Abz-FRLKGGAPIKGV-EDDNP TFAamp;;

Abz-FRLKGGAPIKGV-EDDNP TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Abz-FRLKGGAPIKGV-EDDNP TFA is a fluorogenic substrate used to measure the enzymatic activities of protease forms, such as papain-like protease 2 (PLP2) from severe acute respiratory syndrome coronavirus (SARS-CoV). Abz-FRLKGGAPIKGV-EDDNP TFA has the potential for study 2019-nCoV (COVID-19) infection[1].

体外研究
(In Vitro)

Abz-FRLKGGAPIKGV-EDDNP TFA has little fluorescent emission because of the fluorescence resonance energy transfer (FRET) between Abz and EDDNP. The apparent kcat and Km values for this fluorogenic substrate at pH 7.4 are 20 min-1 and 77 µM, respectively. The enhanced fluorescence emission upon substrate cleavage is monitored at the excitation and emission wavelengths of 320 and 420 nm, respectively, in the presence or absence of the chemical compounds assayed[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Sequence Shortening

Abz-FRLKGGAPIKGV-EDDNP

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Yu-San Han, et al. Papain-like Protease 2 (PLP2) From Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV): Expression, Purification, Characterization, and Inhibition. Biochemistry. 2005 Aug 2;44(30):10349-59.